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KMID : 0380020060210040273
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 4 p.273 ~ p.278
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Microcontact Printing of Bacteria Using Hybrid Agarose Gel Stamp
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Shim Hyun-Woo
Lee Ji-Hye
Lee Chang-Soo
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Abstract
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The noble method of hybrid agarose gel microstamp fabricated by replica molding against PDMS master to make bacteria patterns on agar surface was presented. After the fabricated hybrid agarose gel microstamp was inked with E. coli, we could obtain 2 dimensional bacterial arrays with 50 μm circular spots. And the various shaped patterns based on experimental design were easily generated. The analysis of mean fluorescent signal was showed that bacterial pattern have high contrast between spots and background and homogeneity of pattern. Our proposed method solved the problem of wetting and handling with small soft agarose gel microstamp when bacteria were used for ink. The agarose gel stamp provides appropriate environment to inked bacteria, which is essential technology for cell patterning with high retaining viability during the patterning process. This method is reproducible, convenient, rapid, and could be applied to screening system, study of cell-surface interaction, and microbial ecology.
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KEYWORD
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Bacterial patterning, microcontact printing, agarose gel
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